Bioethics (First Year Seminar)
Infection vs. Immunity (BIOL
Microbiology (BIOL 326)
Virology (BIOL 427)
RNA (BIOL 419)
In my research we work with the plant virus BMV, which is a model RNA virus
(interesting: not everything on Earth has a DNA genome!). Similar human
pathogens include the SARS coronavirus, West Nile virus, and hepatitis C virus.
We use genomic and bioinformatics approaches to identify host factors that are
important in BMV life cycle processes, and use the genetically powerful system
of yeast (see colonies of yeast on plate at left) as a model host. In other work
in the lab, we study in vivo evolution of turnip crinkle virus RNA, trying to
understand the relationship between viral RNA sequence, structure, and function
in infected turnip plants.
Postdoctoral Fellow, University of Wisconsin
Ph.D., University of Pennsylvania,
B.S., Haverford College, 1993
Selected Publications (* indicates student co-author)
Guo, R., *Lin, W., Zhang, J., Simon, A.E., and Kushner, D.B.
(2009). Structural plasticity and rapid evolution in a viral RNA revealed by in
vivo genetic selection. Journal of Virology 83:927-39.
Shan, X., *Russell, T.A., *Paul, S.M., Kushner, D.B., and
Joyce, P.B.M. (2008). Characterization of a temperature-sensitive mutation that
impairs the function of yeast tRNA nucleotidyltransferase. Yeast 25:219-233.
Kushner, D.B. (2007). DNA microarrays in the undergraduate
microbiology lab: experimentation and handling large datasets in as few as six
weeks. Journal of Microbiology & Biology Education 8:3-12.
Ahlquist, P., Schwartz, M., Chen, J., Kushner, D., Hao, L.,
and Dye, B.T. (2005). Viral and host determinants of RNA virus vector
replication and expression. Vaccine 23:1784-1787.
Williams, J.F., Zhang, Y., Williams, M.A., Hou, S., Kushner,
D., and Ricciardi, R.P. (2004). E1A Based Determinants of Oncogenicity
in Human Adenovirus Groups A and C. Current Topics in Microbiology and
Kushner, D.B., Lindenbach, B.D., Grdzelishvili, V.Z.,
Noueiry, A.O., *Paul, S.M., and Ahlquist, P. (2003). Systematic, genome-wide
identification of host genes affecting replication of a positive-strand RNA
virus. Proceedings of the National Academy of Sciences USA 100:15764-15769.
Ahlquist, P., Noueiry, A.O., Lee W.-M., Kushner, D.B., and
Dye, B.T. (2003). Host Factors in Positive-Strand Virus Genome Replication.
Journal of Virology 77:8181-8186.